Hannah Poe peers into a large microscope in the Hector F. DeLuca Biochemical Sciences Building, seeing not just florescent molecules of RNA, but also a future career in biochemistry research.
Poe is about to start her last year in the Department of Biochemistry’s undergraduate program, but it will only be her second year on the University of Wisconsin–Madison campus, as she is a transfer student from a university in Colorado. In addition to her classwork, she became involved in undergraduate research in the department and is currently an undergraduate summer research scholar in Aaron Hoskins’ Lab.
“It’s very beneficial to be able to work in the lab without also taking classes like we do during the fall and spring,” Poe says. “The funding from the scholarship has been very helpful for me securing my position in the summer.”
The Biochemistry Undergraduate Summer Research Scholarships help undergraduates gain focused, full-time research experience early in their academic careers. In return for a summer stipend, students work in the lab for 30 to 40 hours per week for eight weeks during the summer and write a research report on their findings.
Poe actually started working in a lab as soon as she got to campus, eventually working her way to the Hoskins Lab last January and then securing a summer research scholarship. In the lab, she places small molecules that emit light, called fluorophores, on a synthetic strand of RNA, nicknamed Mango. These experiments allow her to characterize how Mango functions as a molecular beacon for lighting up RNAs in cells. The Hoskins Lab is deeply involved with researching the spliceosome, a cellular machine essential for processing precursors to messenger RNA (mRNA) after genes are copied from DNA.
The spliceosome is essential in humans for producing the correct mRNA from genes. These mRNAs ultimately pass along the genetic code in the cell to form proteins needed for function. Mango will someday be used by the Hoskins Lab to discover how different components of the spliceosome work together by allowing researchers to follow RNA movements and interactions in live cells with a microscope.
“[The spliceosome is] really critical in genetics and general cell activity, so the lab is trying to solve the structure and investigate the interactions between different components of this huge multifaceted mechanism,” Poe explains.
In the lab, undergraduates are mentored by the lab’s principal investigator in addition to a postdoctoral scientist or a graduate student. Poe works with Hoskins and postdoctoral scientist, Clarisse van der Feltz. In addition, Hannah shares her results with a Hoskins Lab collaborator on Mango, Peter Unrau of Simon Fraser University in Canada.
“Hannah is very eager to learn and try new things and be independent in the lab,” van der Feltz says. “She is learning to troubleshoot scientific problems to understand how they can be checked and tested.”
Hoskins echoed van der Feltz by adding he is fortunate Poe decided to join his lab. He also says it is extremely important for undergraduates to get involved in research.
“Undergrad research is essential for any student thinking about a career in science,” he says. “It teaches you to work as part of a scientific team, solve problems as an independent scientist, and work on big scientific questions that may take months or years to solve.”
Joe Kraft, a senior in Thomas Record’s lab and another recipient of an Undergraduate Summer Research Scholarship, says the mentor-mentee relationship it provides is extremely beneficial. The basic research happening in the Record Lab has allowed him to gain experience in lab fundamentals essential for a career in research.
“I have been mentored by Professor Record and a graduate student, but this summer I’ve also been able to mentor an undergrad who is new to the lab,” says Kraft, who has been in the Record Lab since his junior year. “Being on both sides of that has been extremely valuable. Those are relationships you have throughout your research life.”
Kraft and Poe are among hundreds of undergraduate researchers on the UW–Madison campus who have benefited from their time in the lab.
Kraft plans to ultimately pursue a Ph.D. in science, with a particular interest in ideas like engineering immune cells to fight cancer. Poe’s summer of research has shown her she enjoys research and may return to graduate school after working in industry for a few years after graduation.
“Being involved in undergraduate research has made me more comfortable in lab and shown me that I enjoy research,” Poe says. “My undergraduate research experience, particularly the time I spent in lab this summer, really formulated my next five years post-graduation, and that’s been invaluable to me.”
This story was originally published on the Department of Biochemistry News site.